Module complementbed added

This commit is contained in:
JoseEspinosa 2020-07-14 15:50:35 +02:00
parent 8155270653
commit f6894c7d3a
8 changed files with 90 additions and 0 deletions

View file

@ -0,0 +1,7 @@
FROM nfcore/base
LABEL authors="Jose Espinosa-Carrasco" \
description="Docker image containing all requirements for nf-core/modules/bedtools/complementbed"
COPY environment.yml /
RUN conda env create -f /environment.yml && conda clean -a
ENV PATH /opt/conda/envs/nf-core-bedtools-complementbed/bin:$PATH

View file

@ -0,0 +1,9 @@
# You can use this file to create a conda environment for this pipeline:
# conda env create -f environment.yml
name: nf-core-bedtools-complementbed
channels:
- conda-forge
- bioconda
- defaults
dependencies:
- bioconda::bedtools=2.29.2

View file

@ -0,0 +1,18 @@
process COMPLEMENT_BED {
tag {input_file}
container 'quay.io/biocontainers/bedtools:2.29.2--hc088bd4_0'
input:
path (input_file)
path (fasta_sizes)
val (complementbed_args)
output:
stdout()
script:
"""
bedtools complement -i ${input_file} -g ${fasta_sizes} ${complementbed_args}
"""
}

View file

@ -0,0 +1,30 @@
name: bedtools complement
description: Returns all intervals in a genome that are not covered by at least one interval in the input BED/GFF/VCF file
keywords:
- complement
tools:
- bedtools:
description: |
Bedtools is a software package that provides with a toolset to perform genome arithmetic operations.
homepage: https://bedtools.readthedocs.io/en/latest/index.html
documentation: https://bedtools.readthedocs.io/en/latest/index.html
doi: 10.093/bioinformatics/btq033
input:
-
- input_file:
type: file
description: Input genomic coordinates file
pattern: "*.{bed,gff,vcf}"
- fasta_sizes:
type: file
description: Genome chromosome sizes
pattern: "*.{txt,sizes}"
output:
-
- index:
type: stdout,file
description:
pattern: "stdout,*.{bed,gff,vcf}"
authors:
- "@JoseEspinosa"

View file

@ -0,0 +1,5 @@
chr1 951 1061
chr1 1300 1420
chr1 1400 1500

View file

@ -0,0 +1 @@
chr1 1780869

View file

@ -0,0 +1,18 @@
#!/usr/bin/env nextflow
nextflow.preview.dsl = 2
params.complementbed_args = ''
include check_output from '../../../../tests/functions/check_process_outputs.nf' // params(params)
include COMPLEMENT_BED from '../main.nf' params(params)
// Define input channels
ch_input = Channel.fromPath('./input_data/A.bed')
chrom_sizes = Channel.fromPath('./input_data/genome.sizes')
// Run the workflow
workflow {
COMPLEMENT_BED(ch_input, chrom_sizes, params.complementbed_args)
// .check_output()
}

View file

@ -0,0 +1,2 @@
docker.enabled = true
params.outdir = './results'