added unaligned output

This commit is contained in:
kevinmenden 2020-12-18 09:14:40 +01:00
parent 41329b8982
commit f840325ead
2 changed files with 57 additions and 42 deletions

View file

@ -26,6 +26,8 @@ process BOWTIE_ALIGN {
tuple val(meta), path('*.bam'), emit: bam
tuple val(meta), path('*.out'), emit: log
path '*.version.txt' , emit: version
tuple val(meta), path('*fastq.gz'), optional:true, emit: fastq
path 'test.txt', emit: test
script:
def software = getSoftwareName(task.process)
@ -45,6 +47,15 @@ process BOWTIE_ALIGN {
2> ${prefix}.out \\
| samtools view $options.args2 -@ $task.cpus -bS -o ${prefix}.bam -
touch test.txt
if [ -f ${prefix}.unmapped.fastq ]; then
gzip ${prefix}.unmapped.fastq
fi
if [ -f ${prefix}.unmapped_1.fastq ]; then
gzip ${prefix}.unmapped_1.fastq
gzip ${prefix}.unmapped_2.fastq
fi
echo \$(bowtie --version 2>&1) | sed 's/^.*bowtie-align-s version //; s/ .*\$//' > ${software}.version.txt
"""
}

View file

@ -7,59 +7,63 @@ keywords:
- reference
tools:
- bowtie:
description: |
bowtie is a software package for mapping DNA sequences against
a large reference genome, such as the human genome.
homepage: http://bowtie-bio.sourceforge.net/index.shtml
documentation: http://bowtie-bio.sourceforge.net/manual.shtml
arxiv: arXiv:1303.3997
description: |
bowtie is a software package for mapping DNA sequences against
a large reference genome, such as the human genome.
homepage: http://bowtie-bio.sourceforge.net/index.shtml
documentation: http://bowtie-bio.sourceforge.net/manual.shtml
arxiv: arXiv:1303.3997
params:
- outdir:
type: string
description: |
The pipeline's output directory. By default, the module will
output files into `$params.outdir/<SOFTWARE>`
type: string
description: |
The pipeline's output directory. By default, the module will
output files into `$params.outdir/<SOFTWARE>`
- publish_dir_mode:
type: string
description: |
Value for the Nextflow `publishDir` mode parameter.
Available: symlink, rellink, link, copy, copyNoFollow, move.
type: string
description: |
Value for the Nextflow `publishDir` mode parameter.
Available: symlink, rellink, link, copy, copyNoFollow, move.
- enable_conda:
type: boolean
description: |
Run the module with Conda using the software specified
via the `conda` directive
type: boolean
description: |
Run the module with Conda using the software specified
via the `conda` directive
- singularity_pull_docker_container:
type: boolean
description: |
Instead of directly downloading Singularity images for use with Singularity,
force the workflow to pull and convert Docker containers instead.
type: boolean
description: |
Instead of directly downloading Singularity images for use with Singularity,
force the workflow to pull and convert Docker containers instead.
- save_unaligned:
type: boolean
description: Save unaligned reads
type: boolean
description: Save unaligned reads
input:
- meta:
type: map
description: |
Groovy Map containing sample information
e.g. [ id:'test', single_end:false ]
type: map
description: |
Groovy Map containing sample information
e.g. [ id:'test', single_end:false ]
- reads:
type: file
description: |
List of input FastQ files of size 1 and 2 for single-end and paired-end data,
respectively.
type: file
description: |
List of input FastQ files of size 1 and 2 for single-end and paired-end data,
respectively.
- index:
type: file
description: Bowtie genome index files
pattern: "*.ebwt"
type: file
description: Bowtie genome index files
pattern: "*.ebwt"
output:
- sam:
type: file
description: Output SAM file containing read alignments
pattern: "*.{sam}"
- bam:
type: file
description: Output SAM file containing read alignments
pattern: "*.{bam}"
- version:
type: file
description: File containing software version
pattern: "*.{version.txt}"
type: file
description: File containing software version
pattern: "*.{version.txt}"
- fastq:
type: file
description: Unaligned FastQ files
pattern: "*.fastq.gz"
authors:
- "@kevinmenden"