process SAMTOOLS_COLLATEFASTQ { tag "$meta.id" label 'process_low' conda (params.enable_conda ? "bioconda::samtools=1.15.1" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/samtools:1.15.1--h1170115_0' : 'quay.io/biocontainers/samtools:1.15.1--h1170115_0' }" input: tuple val(meta), path(input) output: //TODO might be good to have ordered output of the fastq files, so we can // make sure the we get the right files tuple val(meta), path("*_{1,2}.fq.gz"), path("*_other.fq.gz"), path("*_singleton.fq.gz"), emit: reads path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when script: def args = task.ext.args ?: '' def args2 = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" """ samtools collate \\ $args \\ --threads $task.cpus \\ -O \\ $input \\ . | samtools fastq \\ $args2 \\ --threads $task.cpus \\ -1 ${prefix}_1.fq.gz \\ -2 ${prefix}_2.fq.gz \\ -0 ${prefix}_other.fq.gz \\ -s ${prefix}_singleton.fq.gz cat <<-END_VERSIONS > versions.yml "${task.process}": samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') END_VERSIONS """ }