process TRIMMOMATIC { tag "$meta.id" label 'process_medium' conda (params.enable_conda ? "bioconda::trimmomatic=0.39" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/trimmomatic:0.39--hdfd78af_2': 'quay.io/biocontainers/trimmomatic:0.39--hdfd78af_2' }" input: tuple val(meta), path(reads) output: tuple val(meta), path("*.paired.trim*.fastq.gz") , emit: trimmed_reads tuple val(meta), path("*.unpaired.trim_*.fastq.gz"), optional:true, emit: unpaired_reads tuple val(meta), path("*.log") , emit: log path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when script: def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" def trimmed = meta.single_end ? "SE" : "PE" def output = meta.single_end ? "${prefix}.SE.paired.trim.fastq.gz" // HACK to avoid unpaired and paired in the trimmed_reads output : "${prefix}.paired.trim_1.fastq.gz ${prefix}.unpaired.trim_1.fastq.gz ${prefix}.paired.trim_2.fastq.gz ${prefix}.unpaired.trim_2.fastq.gz" // TODO Give better error output def qual_trim = task.ext.args2 ?: '' """ trimmomatic \\ $trimmed \\ -threads $task.cpus \\ -trimlog ${prefix}.log \\ $reads \\ $output \\ $qual_trim \\ $args cat <<-END_VERSIONS > versions.yml "${task.process}": trimmomatic: \$(trimmomatic -version) END_VERSIONS """ }