// Import generic module functions include { initOptions; saveFiles; getSoftwareName } from './functions' params.options = [:] def options = initOptions(params.options) process BCFTOOLS_MPILEUP { tag "$meta.id" publishDir "${params.outdir}", mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), publish_id:meta.id) } conda (params.enable_conda ? "bioconda::bcftools=1.11" : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { container "https://depot.galaxyproject.org/singularity/bcftools:1.11--h7c999a4_0" } else { container "quay.io/biocontainers/bcftools:1.11--h7c999a4_0" } input: tuple val(meta), path(bam) path fasta output: tuple val(meta), path("*.gz") , emit: vcf tuple val(meta), path("*.tbi") , emit: tbi tuple val(meta), path("*stats.txt"), emit: stats path "*.version.txt" , emit: version script: def software = getSoftwareName(task.process) def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" """ echo "${meta.id}" > sample_name.list bcftools mpileup \\ --fasta-ref $fasta \\ $options.args \\ $bam \\ | bcftools call --output-type v $options.args2 \\ | bcftools reheader --samples sample_name.list \\ | bcftools view --output-file ${prefix}.vcf.gz --output-type z $options.args3 tabix -p vcf -f ${prefix}.vcf.gz bcftools stats ${prefix}.vcf.gz > ${prefix}.bcftools_stats.txt echo \$(bcftools --version 2>&1) | sed 's/^.*bcftools //; s/ .*\$//' > ${software}.version.txt """ }