process SRATOOLS_FASTERQDUMP { tag "$meta.id" label 'process_medium' conda (params.enable_conda ? 'bioconda::sra-tools=2.11.0 conda-forge::pigz=2.6' : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/mulled-v2-5f89fe0cd045cb1d615630b9261a1d17943a9b6a:6a9ff0e76ec016c3d0d27e0c0d362339f2d787e6-0' : 'quay.io/biocontainers/mulled-v2-5f89fe0cd045cb1d615630b9261a1d17943a9b6a:6a9ff0e76ec016c3d0d27e0c0d362339f2d787e6-0' }" input: tuple val(meta), path(sra) path ncbi_settings output: tuple val(meta), path(output), emit: reads path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when script: def args = task.ext.args ?: '' def args2 = task.ext.args2 ?: '' // Paired-end data extracted by fasterq-dump (--split-3 the default) always creates // *_1.fastq *_2.fastq files but sometimes also an additional *.fastq file // for unpaired reads which we ignore here. output = meta.single_end ? '*.fastq.gz' : '*_{1,2}.fastq.gz' """ export NCBI_SETTINGS="\$PWD/${ncbi_settings}" fasterq-dump \\ $args \\ --threads $task.cpus \\ ${sra.name} pigz \\ $args2 \\ --no-name \\ --processes $task.cpus \\ *.fastq cat <<-END_VERSIONS > versions.yml "${task.process}": sratools: \$(fasterq-dump --version 2>&1 | grep -Eo '[0-9.]+') pigz: \$( pigz --version 2>&1 | sed 's/pigz //g' ) END_VERSIONS """ }