#!/usr/bin/env nextflow nextflow.enable.dsl = 2 include { BISMARK_GENOMEPREPARATION } from '../../../../software/bismark/genomepreparation/main.nf' addParams( options: [:] ) include { BISMARK_ALIGN as BISMARK_ALIGN_SE } from '../../../../software/bismark/align/main.nf' addParams( options: [ publish_dir:'test_single_end' ] ) include { BISMARK_ALIGN as BISMARK_ALIGN_PE } from '../../../../software/bismark/align/main.nf' addParams( options: [ publish_dir:'test_paired_end' ] ) /* * Test with single-end data */ workflow test_bismark_align_single_end { input = [ [ id:'test', single_end:true ], // meta map [ file("${launchDir}/tests/data/genomics/sarscov2/illumina/fastq/test_methylated_1.fastq.gz", checkIfExists: true) ] ] fasta = file("${launchDir}/tests/data/genomics/sarscov2/genome/genome.fasta", checkIfExists: true) BISMARK_GENOMEPREPARATION ( fasta ) BISMARK_ALIGN_SE ( input, BISMARK_GENOMEPREPARATION.out.index ) } /* * Test with paired-end data */ workflow test_bismark_align_paired_end { input = [ [ id:'test', single_end:false ], // meta map [ file("${launchDir}/tests/data/genomics/sarscov2/illumina/fastq/test_methylated_1.fastq.gz", checkIfExists: true), file("${launchDir}/tests/data/genomics/sarscov2/illumina/fastq/test_methylated_2.fastq.gz", checkIfExists: true) ] ] fasta = file("${launchDir}/tests/data/genomics/sarscov2/genome/genome.fasta", checkIfExists: true) BISMARK_GENOMEPREPARATION ( fasta ) BISMARK_ALIGN_PE ( input, BISMARK_GENOMEPREPARATION.out.index ) }