// Import generic module functions include { initOptions; saveFiles; getSoftwareName } from './functions' params.options = [:] def options = initOptions(params.options) process BWAMEM2_MEM { tag "$meta.id" label 'process_high' publishDir "${params.outdir}", mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), publish_id:meta.id) } conda (params.enable_conda ? "bioconda::bwa-mem2=2.1 bioconda::samtools=1.11" : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { container "https://depot.galaxyproject.org/singularity/mulled-v2-e5d375990341c5aef3c9aff74f96f66f65375ef6:e6f0d20c9d78572ddbbf00d8767ee6ff865edd4e-0" } else { container "quay.io/biocontainers/mulled-v2-e5d375990341c5aef3c9aff74f96f66f65375ef6:e6f0d20c9d78572ddbbf00d8767ee6ff865edd4e-0" } input: tuple val(meta), path(reads) path index output: tuple val(meta), path("*.bam"), emit: bam path "*.version.txt" , emit: version script: def software = getSoftwareName(task.process) def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" def read_group = meta.read_group ? "-R ${meta.read_group}" : "" """ INDEX=`find -L ./ -name "*.amb" | sed 's/.amb//'` bwa-mem2 mem \\ $options.args \\ $read_group \\ -t $task.cpus \\ \$INDEX \\ $reads \\ | samtools view $options.args2 -@ $task.cpus -bhS -o ${prefix}.bam - echo \$(bwa-mem2 version 2>&1) > ${software}.version.txt """ }