process BOWTIE_ALIGN { tag "$meta.id" label 'process_high' conda (params.enable_conda ? 'bioconda::bowtie=1.3.0 bioconda::samtools=1.11' : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/mulled-v2-ffbf83a6b0ab6ec567a336cf349b80637135bca3:9e14e16c284d6860574cf5b624bbc44c793cb024-0' : 'quay.io/biocontainers/mulled-v2-ffbf83a6b0ab6ec567a336cf349b80637135bca3:9e14e16c284d6860574cf5b624bbc44c793cb024-0' }" input: tuple val(meta), path(reads) path index output: tuple val(meta), path('*.bam'), emit: bam tuple val(meta), path('*.out'), emit: log path "versions.yml" , emit: versions tuple val(meta), path('*fastq.gz'), optional:true, emit: fastq script: def args = task.ext.args ?: '' def args2 = task.ext.args2 ?: '' def prefix = task.ext.suffix ? "${meta.id}${task.ext.suffix}" : "${meta.id}" def unaligned = params.save_unaligned ? "--un ${prefix}.unmapped.fastq" : '' def endedness = meta.single_end ? "$reads" : "-1 ${reads[0]} -2 ${reads[1]}" """ INDEX=`find -L ./ -name "*.3.ebwt" | sed 's/.3.ebwt//'` bowtie \\ --threads $task.cpus \\ --sam \\ -x \$INDEX \\ -q \\ $unaligned \\ $args \\ $endedness \\ 2> ${prefix}.out \\ | samtools view $args2 -@ $task.cpus -bS -o ${prefix}.bam - if [ -f ${prefix}.unmapped.fastq ]; then gzip ${prefix}.unmapped.fastq fi if [ -f ${prefix}.unmapped_1.fastq ]; then gzip ${prefix}.unmapped_1.fastq gzip ${prefix}.unmapped_2.fastq fi cat <<-END_VERSIONS > versions.yml "${task.process}": bowtie: \$(echo \$(bowtie --version 2>&1) | sed 's/^.*bowtie-align-s version //; s/ .*\$//') samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') END_VERSIONS """ }