// Import generic module functions include { initOptions; saveFiles; getSoftwareName } from './functions' // TODO nf-core: All of these TODO statements can be deleted after the relevant changes have been made. // TODO nf-core: If in doubt look at other nf-core/modules to see how we are doing things! :) // https://github.com/nf-core/modules/tree/master/software // You can also ask for help via your pull request or on the #modules channel on the nf-core Slack workspace: // https://nf-co.re/join // TODO nf-core: The key words "MUST", "MUST NOT", "SHOULD", etc. are to be interpreted as described in RFC 2119 (https://tools.ietf.org/html/rfc2119). // TODO nf-core: A module file SHOULD only define input and output files as command-line parameters. // All other parameters MUST be provided as a string i.e. "options.args" // where "params.options" is a Groovy Map that MUST be provided via the addParams section of the including workflow. // Any parameters that need to be evaluated in the context of a particular sample // e.g. single-end/paired-end data MUST also be defined and evaluated appropriately. // TODO nf-core: Software that can be piped together SHOULD be added to separate module files // unless there is a run-time, storage advantage in implementing in this way // e.g. bwa mem | samtools view -B -T ref.fasta to output BAM instead of SAM. // TODO nf-core: Optional inputs are not currently supported by Nextflow. However, "fake files" MAY be used to work around this issue. params.options = [:] def options = initOptions(params.options) // TODO nf-core: Process name MUST be all uppercase, // "TOOL" and (ideally) "SUBTOOL" MUST be all one word separated by an "_". process TOOL_SUBTOOL { // TODO nf-core: If a meta map of sample information is NOT provided in "input:" section // change tag value to another appropriate input value e.g. tag "$fasta" tag "$meta.id" // TODO nf-core: Provide appropriate resource label for process as listed in the nf-core pipeline template below: // https://github.com/nf-core/tools/blob/master/nf_core/pipeline-template/%7B%7Bcookiecutter.name_noslash%7D%7D/conf/base.config#L29 label 'process_medium' publishDir "${params.outdir}", mode: params.publish_dir_mode, // TODO nf-core: If a meta map of sample information is NOT provided in "input:" section // change "publish_id:meta.id" to initialise an empty string e.g. "publish_id:''". saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), publish_id:meta.id) } // TODO nf-core: List required Conda packages. // Software MUST be pinned to channel (i.e. "bioconda"), version (i.e. "1.10") and build (i.e. "h9402c20_2") as in the example below. conda (params.enable_conda ? "bioconda::samtools=1.10=h9402c20_2" : null) // TODO nf-core: See section in main README for further information regarding finding and adding container addresses to the section below. if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { container "https://depot.galaxyproject.org/singularity/samtools:1.10--h9402c20_2" } else { container "quay.io/biocontainers/samtools:1.10--h9402c20_2" } input: // TODO nf-core: Where applicable all sample-specific information e.g. "id", "single_end", "read_group" // MUST be provided as an input via a Groovy Map called "meta". // This information may not be required in some instances e.g. indexing reference genome files: // https://github.com/nf-core/modules/blob/master/software/bwa/index/main.nf // TODO nf-core: Where applicable please provide/convert compressed files as input/output // e.g. "*.fastq.gz" and NOT "*.fastq", "*.bam" and NOT "*.sam" etc. tuple val(meta), path(bam) output: // TODO nf-core: Named file extensions MUST be emitted for ALL output channels // TODO nf-core: If meta is provided in "input:" section then it MUST be added to ALL output channels (except version) tuple val(meta), path("*.bam"), emit: bam // TODO nf-core: List additional required output channels/values here path "*.version.txt" , emit: version script: def software = getSoftwareName(task.process) // TODO nf-core: If a meta map of sample information is NOT provided in "input:" section delete the line below def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" // TODO nf-core: Where possible, a command MUST be provided to obtain the version number of the software e.g. 1.10 // If the software is unable to output a version number on the command-line then it can be manually specified // e.g. https://github.com/nf-core/modules/blob/master/software/homer/annotatepeaks/main.nf // TODO nf-core: It MUST be possible to pass additional parameters to the tool as a command-line string via the "$options.args" variable // TODO nf-core: If the tool supports multi-threading then you MUST provide the appropriate parameter // using the Nextflow "task" variable e.g. "--threads $task.cpus" // TODO nf-core: Please indent the command appropriately (4 spaces!!) to help with readability ;) """ samtools \\ sort \\ $options.args \\ -@ $task.cpus \\ -o ${prefix}.bam \\ -T $prefix \\ $bam echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//' > ${software}.version.txt """ }