// Import generic module functions include { initOptions; saveFiles; getSoftwareName } from './functions' params.options = [:] options = initOptions(params.options) process STRINGTIE { tag "$meta.id" label 'process_medium' publishDir "${params.outdir}", mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } conda (params.enable_conda ? "bioconda::stringtie=2.1.4" : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { container "https://depot.galaxyproject.org/singularity/stringtie:2.1.4--h7e0af3c_0" } else { container "quay.io/biocontainers/stringtie:2.1.4--h7e0af3c_0" } input: tuple val(meta), path(bam) path gtf output: tuple val(meta), path("*.coverage.gtf") , emit: coverage_gtf tuple val(meta), path("*.transcripts.gtf"), emit: transcript_gtf tuple val(meta), path("*.abundance.txt") , emit: abundance tuple val(meta), path("*.ballgown") , emit: ballgown path "*.version.txt" , emit: version script: def software = getSoftwareName(task.process) def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" def strandedness = '' if (meta.strandedness == 'forward') { strandedness = '--fr' } else if (meta.strandedness == 'reverse') { strandedness = '--rf' } """ stringtie \\ $bam \\ $strandedness \\ -G $gtf \\ -o ${prefix}.transcripts.gtf \\ -A ${prefix}.gene.abundance.txt \\ -C ${prefix}.coverage.gtf \\ -b ${prefix}.ballgown \\ $options.args stringtie --version > ${software}.version.txt """ }