// Import generic module functions include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' params.options = [:] options = initOptions(params.options) process GATK4_MUTECT2 { tag "$meta.id" label 'process_medium' publishDir "${params.outdir}", mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } conda (params.enable_conda ? "bioconda::gatk4=4.2.0.0" : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { container "https://depot.galaxyproject.org/singularity/gatk4:4.2.0.0--0" } else { container "quay.io/biocontainers/gatk4:4.2.0.0--0" } input: tuple val(meta) , path(bam) , path(bai) , val(which_norm) val run_single val run_pon path fasta path fastaidx path dict path germline_resource path germline_resource_idx path panel_of_normals path panel_of_normals_idx output: tuple val(meta), path("*.vcf.gz") , emit: vcf tuple val(meta), path("*.tbi") , emit: tbi tuple val(meta), path("*.stats") , emit: stats tuple val(meta), path("*.f1r2.tar.gz"), optional:true, emit: f1r2 path "versions.yml" , emit: versions script: def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" def inputsList = [] def normalsList = [] def inputsCommand = '' def panelsCommand = '' def normalsCommand = '' bam.each() {a -> inputsList.add(" -I " + a ) } inputsCommand = inputsList.join( ' ') if(run_pon) { panelsCommand = '' normalsCommand = '' } else if(run_single) { panelsCommand = " --germline-resource $germline_resource --panel-of-normals $panel_of_normals" normalsCommand = '' } else { panelsCommand = " --germline-resource $germline_resource --panel-of-normals $panel_of_normals --f1r2-tar-gz ${prefix}.f1r2.tar.gz" which_norm.each() {a -> normalsList.add(" -normal " + a ) } normalsCommand = normalsList.join( ' ') } """ gatk Mutect2 \\ -R ${fasta} \\ ${inputsCommand} \\ ${normalsCommand} \\ ${panelsCommand} \\ -O ${prefix}.vcf.gz \\ $options.args cat <<-END_VERSIONS > versions.yml ${getProcessName(task.process)}: ${getSoftwareName(task.process)}: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//') END_VERSIONS """ }