// Import generic module functions include { initOptions; saveFiles; getSoftwareName } from './functions' params.options = [:] options = initOptions(params.options) process BOWTIE2_ALIGN { tag "$meta.id" label 'process_high' publishDir "${params.outdir}", mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), publish_id:meta.id) } conda (params.enable_conda ? 'bioconda::bowtie2=2.4.2 bioconda::samtools=1.11 conda-forge::pigz=2.3.4' : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { container "https://depot.galaxyproject.org/singularity/mulled-v2-ac74a7f02cebcfcc07d8e8d1d750af9c83b4d45a:577a697be67b5ae9b16f637fd723b8263a3898b3-0" } else { container "quay.io/biocontainers/mulled-v2-ac74a7f02cebcfcc07d8e8d1d750af9c83b4d45a:577a697be67b5ae9b16f637fd723b8263a3898b3-0" } input: tuple val(meta), path(reads) path index output: tuple val(meta), path('*.bam'), emit: bam tuple val(meta), path('*.log'), emit: log path '*.version.txt' , emit: version tuple val(meta), path('*fastq.gz'), optional:true, emit: fastq script: def software = getSoftwareName(task.process) def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" if (meta.single_end) { def unaligned = params.save_unaligned ? "--un-gz ${prefix}.unmapped.fastq.gz" : '' """ INDEX=`find -L ./ -name "*.rev.1.bt2" | sed 's/.rev.1.bt2//'` bowtie2 \\ -x \$INDEX \\ -U $reads \\ --threads $task.cpus \\ $unaligned \\ $options.args \\ 2> ${prefix}.bowtie2.log \\ | samtools view -@ $task.cpus $options.args2 -bhS -o ${prefix}.bam - echo \$(bowtie2 --version 2>&1) | sed 's/^.*bowtie2-align-s version //; s/ .*\$//' > ${software}.version.txt """ } else { def unaligned = params.save_unaligned ? "--un-conc-gz ${prefix}.unmapped.fastq.gz" : '' """ INDEX=`find -L ./ -name "*.rev.1.bt2" | sed 's/.rev.1.bt2//'` bowtie2 \\ -x \$INDEX \\ -1 ${reads[0]} \\ -2 ${reads[1]} \\ --threads $task.cpus \\ $unaligned \\ $options.args \\ 2> ${prefix}.bowtie2.log \\ | samtools view -@ $task.cpus $options.args2 -bhS -o ${prefix}.bam - if [ -f ${prefix}.unmapped.fastq.1.gz ]; then mv ${prefix}.unmapped.fastq.1.gz ${prefix}.unmapped_1.fastq.gz fi if [ -f ${prefix}.unmapped.fastq.2.gz ]; then mv ${prefix}.unmapped.fastq.2.gz ${prefix}.unmapped_2.fastq.gz fi echo \$(bowtie2 --version 2>&1) | sed 's/^.*bowtie2-align-s version //; s/ .*\$//' > ${software}.version.txt """ } }