process GATK4_MARKDUPLICATES_SPARK { tag "$meta.id" label 'process_high' conda (params.enable_conda ? "bioconda::gatk4=4.2.3.0" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/gatk4:4.2.3.0--hdfd78af_0' : 'broadinstitute/gatk:4.2.3.0' }" input: tuple val(meta), path(bam) path fasta path fasta_fai path dict output: tuple val(meta), path("${prefix}"), emit: output path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when script: def args = task.ext.args ?: '' prefix = task.ext.prefix ?: "${meta.id}" def input_list = bam.collect{"--input $it"}.join(' ') def avail_mem = 3 if (!task.memory) { log.info '[GATK MarkDuplicatesSpark] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.' } else { avail_mem = task.memory.giga } """ export SPARK_USER=spark3 gatk --java-options "-Xmx${avail_mem}g" MarkDuplicatesSpark \\ $input_list \\ --output $prefix \\ --reference $fasta \\ --spark-master local[${task.cpus}] \\ --tmp-dir . \\ $args cat <<-END_VERSIONS > versions.yml "${task.process}": gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//') END_VERSIONS """ }