process PICARD_COLLECTMULTIPLEMETRICS { tag "$meta.id" label 'process_medium' conda (params.enable_conda ? "bioconda::picard=2.27.4" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/picard:2.27.4--hdfd78af_0' : 'quay.io/biocontainers/picard:2.27.4--hdfd78af_0' }" input: tuple val(meta), path(bam) path fasta path fai output: tuple val(meta), path("*_metrics"), emit: metrics tuple val(meta), path("*.pdf") , emit: pdf path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when script: def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" def reference = fasta ? "--REFERENCE_SEQUENCE ${fasta}" : "" def avail_mem = 3 if (!task.memory) { log.info '[Picard CollectMultipleMetrics] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.' } else { avail_mem = task.memory.giga } """ picard \\ -Xmx${avail_mem}g \\ CollectMultipleMetrics \\ $args \\ --INPUT $bam \\ --OUTPUT ${prefix}.CollectMultipleMetrics \\ $reference cat <<-END_VERSIONS > versions.yml "${task.process}": picard: \$(picard CollectMultipleMetrics --version 2>&1 | grep -o 'Version.*' | cut -f2- -d:) END_VERSIONS """ stub: def prefix = task.ext.prefix ?: "${meta.id}" """ touch ${prefix}.CollectMultipleMetrics.alignment_summary_metrics touch ${prefix}.CollectMultipleMetrics.insert_size_metrics touch ${prefix}.CollectMultipleMetrics.quality_distribution.pdf touch ${prefix}.CollectMultipleMetrics.base_distribution_by_cycle_metrics touch ${prefix}.CollectMultipleMetrics.quality_by_cycle_metrics touch ${prefix}.CollectMultipleMetrics.read_length_histogram.pdf touch ${prefix}.CollectMultipleMetrics.base_distribution_by_cycle.pdf touch ${prefix}.CollectMultipleMetrics.quality_by_cycle.pdf touch ${prefix}.CollectMultipleMetrics.insert_size_histogram.pdf touch ${prefix}.CollectMultipleMetrics.quality_distribution_metrics cat <<-END_VERSIONS > versions.yml "${task.process}": picard: \$(echo \$(picard CollectMultipleMetrics --version 2>&1) | grep -o 'Version:.*' | cut -f2- -d:) END_VERSIONS """ }