process FAQCS { tag "$meta.id" label 'process_medium' conda (params.enable_conda ? "bioconda::faqcs=2.10" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/faqcs%3A2.10--r41h9a82719_2' : 'quay.io/biocontainers/faqcs:2.10--r41h9a82719_2' }" input: tuple val(meta), path(reads) output: tuple val(meta), path('*.trimmed.fastq.gz') , emit: reads tuple val(meta), path('*.stats.txt') , emit: stats tuple val(meta), path('*.txt') , optional:true, emit: txt tuple val(meta), path('*_qc_report.pdf') , optional:true, emit: statspdf tuple val(meta), path('*.log') , emit: log tuple val(meta), path('*.discard.fastq.gz') , optional:true, emit: reads_fail tuple val(meta), path('*.trimmed.unpaired.fastq.gz') , optional:true, emit: reads_unpaired path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when script: def args = task.ext.args ?: '' // Added soft-links to original fastqs for consistent naming in MultiQC def prefix = task.ext.prefix ?: "${meta.id}" if (meta.single_end) { """ [ ! -f ${prefix}.fastq.gz ] && ln -s $reads ${prefix}.fastq.gz FaQCs \\ -d . \\ -u ${prefix}.fastq.gz \\ --prefix ${prefix} \\ -t $task.cpus \\ $args \\ 2> ${prefix}.fastp.log if [[ -f ${prefix}.unpaired.trimmed.fastq ]]; then mv ${prefix}.unpaired.trimmed.fastq ${prefix}.trimmed.fastq gzip ${prefix}.trimmed.fastq fi if [[ -f ${prefix}.discard.trimmed.fastq ]]; then mv ${prefix}.discard.trimmed.fastq ${prefix}.trimmed.discard.fastq gzip ${prefix}.trimmed.discard.fastq fi cat <<-END_VERSIONS > versions.yml "${task.process}": faqcs: \$(echo \$(FaQCs --version 2>&1) | sed 's/^.*Version: //;' ) END_VERSIONS """ } else { """ [ ! -f ${prefix}_1.fastq.gz ] && ln -s ${reads[0]} ${prefix}_1.fastq.gz [ ! -f ${prefix}_2.fastq.gz ] && ln -s ${reads[1]} ${prefix}_2.fastq.gz FaQCs \\ -d . \\ -1 ${prefix}_1.fastq.gz \\ -2 ${prefix}_2.fastq.gz \\ --prefix ${meta.id} \\ -t $task.cpus \\ $args \\ 2> ${prefix}.fastp.log # Unpaired if [[ -f ${prefix}.unpaired.trimmed.fastq ]]; then # If it is empty remove it if [[ ! -s ${prefix}.unpaired.trimmed.fastq ]]; then rm ${prefix}.unpaired.trimmed.fastq else mv ${prefix}.unpaired.trimmed.fastq ${prefix}.trimmed.unpaired.fastq gzip ${prefix}.trimmed.unpaired.fastq fi fi # R1 if [[ -f ${prefix}.1.trimmed.fastq ]]; then mv ${prefix}.1.trimmed.fastq ${prefix}_1.trimmed.fastq gzip ${prefix}_1.trimmed.fastq fi # R2 if [[ -f ${prefix}.2.trimmed.fastq ]]; then mv ${prefix}.2.trimmed.fastq ${prefix}_2.trimmed.fastq gzip ${prefix}_2.trimmed.fastq fi # Discarded: Created if --discard argument is passed if [[ -f ${prefix}.discard.trimmed.fastq ]]; then mv ${prefix}.discard.trimmed.fastq ${prefix}.trimmed.discard.fastq gzip ${prefix}.trimmed.discard.fastq fi cat <<-END_VERSIONS > versions.yml "${task.process}": faqcs: \$(echo \$(FaQCs --version 2>&1) | sed 's/^.*Version: //;' ) END_VERSIONS """ } }