// Import generic module functions include { initOptions; saveFiles; getSoftwareName } from './functions' process SUBREAD_FEATURECOUNTS { tag "$meta.id" label 'process_medium' publishDir "${params.outdir}", mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:options, publish_dir:getSoftwareName(task.process), publish_id:meta.id) } container "quay.io/biocontainers/subread:2.0.1--hed695b0_0" //container "https://depot.galaxyproject.org/singularity/subread:2.0.1--hed695b0_0" conda (params.conda ? "bioconda::subread=2.0.1" : null) input: tuple val(meta), path(bams), path(annotation) val options output: tuple val(meta), path("*featureCounts.txt") , emit: counts tuple val(meta), path("*featureCounts.txt.summary"), emit: summary path "*.version.txt" , emit: version script: def software = getSoftwareName(task.process) def ioptions = initOptions(options) def prefix = ioptions.suffix ? "${meta.id}${ioptions.suffix}" : "${meta.id}" def paired_end = meta.single_end ? '' : '-p' def strandedness = 0 if (meta.strandedness == 'forward') { strandedness = 1 } else if (meta.strandedness == 'reverse') { strandedness = 2 } """ featureCounts \\ $ioptions.args \\ $paired_end \\ -T $task.cpus \\ -a $annotation \\ -s $strandedness \\ -o ${prefix}.featureCounts.txt \\ ${bams.join(' ')} echo \$(featureCounts -v 2>&1) | sed -e "s/featureCounts v//g" > ${software}.version.txt """ }