params.bwa_options = "-M -B 2" params.sequencer = "ILLUMINA" process bwa_mem { tag "$id" publishDir "${params.outdir}/bwa_mem", mode: 'copy' //TO-DO: Change container declaration, for now a test container is present in my personal docker acccount container 'jeremy1805/bwa-mem-img' input: tuple val(id), path(reads) path genomeindex val indexprefix output: tuple path("*.bam"), path("*.bai") script: """ bwa mem -t ${task.cpus} -R "@RG\\tID:${id}\\tLB:${id}\\tSM:${id}\\tPL:${params.sequencer}" \\ ${params.bwa_options} ${indexprefix} ${reads} | samtools sort -@8 -O BAM -o ${id}.bam - samtools index ${id}.bam """ }