// Import generic module functions include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' params.options = [:] options = initOptions(params.options) process MANTA_GERMLINE { tag "$meta.id" label 'process_high' publishDir "${params.outdir}", mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } conda (params.enable_conda ? "bioconda::manta=1.6.0" : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { container "https://depot.galaxyproject.org/singularity/manta:1.6.0--h9ee0642_1" } else { container "quay.io/biocontainers/manta:1.6.0--h9ee0642_1" } input: tuple val(meta), path(cram), path(crai) path fasta path fai path target_bed path target_bed_tbi output: tuple val(meta), path("*candidate_small_indels.vcf.gz") , emit: candidate_small_indels_vcf tuple val(meta), path("*candidate_small_indels.vcf.gz.tbi"), emit: candidate_small_indels_vcf_tbi tuple val(meta), path("*candidate_sv.vcf.gz") , emit: candidate_sv_vcf tuple val(meta), path("*candidate_sv.vcf.gz.tbi") , emit: candidate_sv_vcf_tbi tuple val(meta), path("*diploid_sv.vcf.gz") , emit: diploid_sv_vcf tuple val(meta), path("*diploid_sv.vcf.gz.tbi") , emit: diploid_sv_vcf_tbi path "versions.yml" , emit: versions script: def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" def options_manta = target_bed ? "--exome --callRegions $target_bed" : "" """ configManta.py \ --bam $cram \ --reference $fasta \ $options_manta \ --runDir manta python manta/runWorkflow.py -m local -j $task.cpus mv manta/results/variants/candidateSmallIndels.vcf.gz \ ${prefix}.candidate_small_indels.vcf.gz mv manta/results/variants/candidateSmallIndels.vcf.gz.tbi \ ${prefix}.candidate_small_indels.vcf.gz.tbi mv manta/results/variants/candidateSV.vcf.gz \ ${prefix}.candidate_sv.vcf.gz mv manta/results/variants/candidateSV.vcf.gz.tbi \ ${prefix}.candidate_sv.vcf.gz.tbi mv manta/results/variants/diploidSV.vcf.gz \ ${prefix}.diploid_sv.vcf.gz mv manta/results/variants/diploidSV.vcf.gz.tbi \ ${prefix}.diploid_sv.vcf.gz.tbi cat <<-END_VERSIONS > versions.yml ${getProcessName(task.process)}: ${getSoftwareName(task.process)}: \$( configManta.py --version ) END_VERSIONS """ }