// Import generic module functions include { initOptions; saveFiles; getSoftwareName } from './functions' params.options = [:] def options = initOptions(params.options) process SUBREAD_FEATURECOUNTS { tag "$meta.id" label 'process_medium' publishDir "${params.outdir}", mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), publish_id:meta.id) } // Note: 2.7X indices incompatible with AWS iGenomes. conda (params.enable_conda ? "bioconda::subread=2.0.1" : null) container "quay.io/biocontainers/subread:2.0.1--hed695b0_0" input: tuple val(meta), path(bams), path(annotation) output: tuple val(meta), path("*featureCounts.txt") , emit: counts tuple val(meta), path("*featureCounts.txt.summary"), emit: summary path "*.version.txt" , emit: version script: def software = getSoftwareName(task.process) def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" def paired_end = meta.single_end ? '' : '-p' def strandedness = 0 if (meta.strandedness == 'forward') { strandedness = 1 } else if (meta.strandedness == 'reverse') { strandedness = 2 } """ featureCounts \\ $options.args \\ $paired_end \\ -T $task.cpus \\ -a $annotation \\ -s $strandedness \\ -o ${prefix}.featureCounts.txt \\ ${bams.join(' ')} echo \$(featureCounts -v 2>&1) | sed -e "s/featureCounts v//g" > ${software}.version.txt """ }