include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' params.options = [:] options = initOptions(params.options) process ADAPTERREMOVAL { tag "$meta.id" label 'process_medium' publishDir "${params.outdir}", mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } conda (params.enable_conda ? "bioconda::adapterremoval=2.3.2" : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { container "https://depot.galaxyproject.org/singularity/adapterremoval:2.3.2--hb7ba0dd_0" } else { container "quay.io/biocontainers/adapterremoval:2.3.2--hb7ba0dd_0" } input: tuple val(meta), path(reads) output: tuple val(meta), path('*.fastq.gz'), emit: reads tuple val(meta), path('*.log') , emit: log path "versions.yml" , emit: versions script: def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" if (meta.single_end) { """ AdapterRemoval \\ --file1 $reads \\ $options.args \\ --basename $prefix \\ --threads $task.cpus \\ --settings ${prefix}.log \\ --output1 ${prefix}.trimmed.fastq.gz \\ --seed 42 \\ --gzip \\ cat <<-END_VERSIONS > versions.yml ${getProcessName(task.process)}: ${getSoftwareName(task.process)}: \$(AdapterRemoval --version 2>&1 | sed -e "s/AdapterRemoval ver. //g") END_VERSIONS """ } else if (!meta.single_end && !meta.collapse) { """ AdapterRemoval \\ --file1 ${reads[0]} \\ --file2 ${reads[1]} \\ $options.args \\ --basename $prefix \\ --threads $task.cpus \\ --settings ${prefix}.log \\ --output1 ${prefix}.pair1.trimmed.fastq.gz \\ --output2 ${prefix}.pair2.trimmed.fastq.gz \\ --seed 42 \\ --gzip \\ cat <<-END_VERSIONS > versions.yml ${getProcessName(task.process)}: ${getSoftwareName(task.process)}: \$(AdapterRemoval --version 2>&1 | sed -e "s/AdapterRemoval ver. //g") END_VERSIONS """ } else { """ AdapterRemoval \\ --file1 ${reads[0]} \\ --file2 ${reads[1]} \\ --collapse \\ $options.args \\ --basename $prefix \\ --threads $task.cpus \\ --settings ${prefix}.log \\ --seed 42 \\ --gzip \\ cat *.collapsed.gz *.collapsed.truncated.gz > ${prefix}.merged.fastq.gz cat <<-END_VERSIONS > versions.yml ${getProcessName(task.process)}: ${getSoftwareName(task.process)}: \$(AdapterRemoval --version 2>&1 | sed -e "s/AdapterRemoval ver. //g") END_VERSIONS """ } }