#!/usr/bin/env nextflow nextflow.enable.dsl = 2 include { UNTAR } from '../../../../modules/untar/main.nf' include { KRAKEN2_KRAKEN2 } from '../../../../modules/kraken2/kraken2/main.nf' include { BRACKEN_BRACKEN } from '../../../../modules/bracken/bracken/main.nf' workflow test_bracken_bracken_single_end_default_args { input = [ [ id:'test', single_end:true ], // meta map [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) ] ] db = file(params.test_data['sarscov2']['genome']['kraken2_bracken_tar_gz'], checkIfExists: true) ch_db = UNTAR ( [[:], db] ).untar .map { it[1] } KRAKEN2_KRAKEN2 ( input, ch_db ) BRACKEN_BRACKEN ( KRAKEN2_KRAKEN2.out.txt, ch_db ) } workflow test_bracken_bracken_single_end_custom_args { input = [ [ id:'test', single_end:true, threshold:0, taxonomic_level:'G', read_length:100 ], // meta map [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) ] ] db = file(params.test_data['sarscov2']['genome']['kraken2_bracken_tar_gz'], checkIfExists: true) ch_db = UNTAR ( [[:], db] ).untar .map { it[1] } KRAKEN2_KRAKEN2 ( input, ch_db ) BRACKEN_BRACKEN ( KRAKEN2_KRAKEN2.out.txt, ch_db ) } workflow test_bracken_bracken_paired_end_default_args { input = [ [ id:'test', single_end:false ], // meta map [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) ] ] db = file(params.test_data['sarscov2']['genome']['kraken2_bracken_tar_gz'], checkIfExists: true) ch_db = UNTAR ( [[:], db] ).untar .map { it[1] } KRAKEN2_KRAKEN2 ( input, ch_db ) BRACKEN_BRACKEN ( KRAKEN2_KRAKEN2.out.txt, ch_db ) } workflow test_bracken_bracken_paired_end_custom_args { input = [ [ id:'test', single_end:false, threshold:0, taxonomic_level:'G', read_length:100 ], // meta map [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) ] ] db = file(params.test_data['sarscov2']['genome']['kraken2_bracken_tar_gz'], checkIfExists: true) ch_db = UNTAR ( [[:], db] ).untar .map { it[1] } KRAKEN2_KRAKEN2 ( input, ch_db ) BRACKEN_BRACKEN ( KRAKEN2_KRAKEN2.out.txt, ch_db ) }