// Import generic module functions include { initOptions; saveFiles; getSoftwareName } from './functions' params.options = [:] def options = initOptions(params.options) process STRINGTIE { tag "$meta.id" label 'process_medium' publishDir "${params.outdir}", mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), publish_id:meta.id) } // Note: 2.7X indices incompatible with AWS iGenomes. conda (params.enable_conda ? "bioconda::stringtie=2.1.4" : null) container "quay.io/biocontainers/stringtie:2.1.4--h7e0af3c_0" input: tuple val(meta), path(bam) path gtf output: tuple val(meta), path("*.coverage.gtf") , emit: coverage_gtf tuple val(meta), path("*.transcripts.gtf"), emit: transcript_gtf tuple val(meta), path("*.txt") , emit: abundance tuple val(meta), path("*.ballgown") , emit: ballgown path "*.version.txt" , emit: version script: def software = getSoftwareName(task.process) def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" def strandedness = '' if (meta.strandedness == 'forward') { strandedness = '--fr' } else if (meta.strandedness == 'reverse') { strandedness = '--rf' } """ stringtie \\ $bam \\ $strandedness \\ -G $gtf \\ -o ${prefix}.transcripts.gtf \\ -A ${prefix}.gene_abundance.txt \\ -C ${prefix}.coverage.gtf \\ -b ${prefix}.ballgown \\ $options.args stringtie --version > ${software}.version.txt """ }