// Import generic module functions include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' params.options = [:] options = initOptions(params.options) process PBCCS { tag "$meta.id" label 'process_low' publishDir "${params.outdir}", mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } conda (params.enable_conda ? "bioconda::pbccs=6.0.0" : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { container "https://depot.galaxyproject.org/singularity/pbccs:6.0.0--h9ee0642_2" } else { container "quay.io/biocontainers/pbccs:6.0.0--h9ee0642_2" } input: tuple val(meta), path(bam), path(pbi) val chunk_num val chunk_on output: tuple val(meta), path("*.ccs.bam") , emit: bam tuple val(meta), path("*.ccs.bam.pbi") , emit: pbi tuple val(meta), path("*.ccs_report.txt" ) , emit: ccs_report_txt tuple val(meta), path("*.ccs_report.json" ) , emit: ccs_report_json tuple val(meta), path("*.zmw_metrics.json.gz"), emit: zmw_metrics path "versions.yml" , emit: versions script: def ccs = bam.toString().replaceAll(/bam$/, '') + chunk_num + '.ccs.bam' def report_txt = bam.toString().replaceAll(/bam$/, '') + chunk_num + '.ccs_report.txt' def report_json = bam.toString().replaceAll(/bam$/, '') + chunk_num + '.ccs_report.json' def zmw_metrics = bam.toString().replaceAll(/bam$/, '') + chunk_num + '.zmw_metrics.json.gz' """ ccs \\ $bam \\ $ccs \\ --report-file $report_txt \\ --report-json $report_json \\ --metrics-json $zmw_metrics \\ --chunk $chunk_num/$chunk_on \\ -j $task.cpus \\ $options.args cat <<-END_VERSIONS > versions.yml ${getProcessName(task.process)}: ${getSoftwareName(task.process)}: \$(echo \$(ccs --version 2>&1) | grep 'ccs' | sed 's/^.*ccs //; s/ .*\$//') END_VERSIONS """ }