// Import generic module functions include { initOptions; saveFiles; getSoftwareName } from './functions' params.options = [:] options = initOptions(params.options) process STRELKA_GERMLINE { tag "$meta.id" label 'process_high' publishDir "${params.outdir}", mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), publish_id:meta.id) } conda (params.enable_conda ? "bioconda::strelka=2.9.10" : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { container "https://depot.galaxyproject.org/singularity/strelka:2.9.10--0" } else { container "quay.io/biocontainers/strelka:2.9.10--0" } input: tuple val(meta), path(bam), path(bai) path fasta path fai path target_bed output: tuple val(meta), path("*_variants.vcf.gz"), emit: vcf tuple val(meta), path("*_variants.vcf.gz.tbi"), emit: vcf_tbi tuple val(meta), path("*_genome.vcf.gz"), emit: genome_vcf tuple val(meta), path("*_genome.vcf.gz.tbi"), emit: genome_vcf_tbi path "*.version.txt", emit: version script: def software = getSoftwareName(task.process) def ioptions = initOptions(options) def prefix = ioptions.suffix ? "strelka_${meta.id}${ioptions.suffix}" : "strelka_${meta.id}" options_strelka = params.target_bed ? "--exome --callRegions ${target_bed}" : "" """ configureStrelkaGermlineWorkflow.py \ --bam ${bam} \ --referenceFasta ${fasta} \ ${options_strelka} \ ${options.args} \ --runDir strelka python strelka/runWorkflow.py -m local -j ${task.cpus} mv strelka/results/variants/genome.*.vcf.gz ${prefix}_genome.vcf.gz mv strelka/results/variants/genome.*.vcf.gz.tbi ${prefix}_genome.vcf.gz.tbi mv strelka/results/variants/variants.vcf.gz ${prefix}_variants.vcf.gz mv strelka/results/variants/variants.vcf.gz.tbi ${prefix}_variants.vcf.gz.tbi echo configureStrelkaGermlineWorkflow.py --version &> ${software}.version.txt #2>&1 """ }