name: seacr_callpeak description: Call peaks using SEACR on sequenced reads in bedgraph format keywords: - peak-caller - peaks - bedgraph - cut&tag - cut&run - chromatin - seacr tools: - seacr: description: | SEACR is intended to call peaks and enriched regions from sparse CUT&RUN or chromatin profiling data in which background is dominated by "zeroes" (i.e. regions with no read coverage). homepage: https://github.com/FredHutch/SEACR documentation: https://github.com/FredHutch/SEACR licence: ["GPL-2.0-only"] input: - meta: type: map description: | Groovy Map containing sample information e.g. [ id:'test', single_end:false ] - bedgraph: type: file description: | The target bedgraph file from which the peaks will be calculated. - ctrlbedgraph: type: file description: | Control (IgG) data bedgraph file to generate an empirical threshold for peak calling. - threshold: type: value description: | Threshold value used to call peaks if the ctrlbedgraph input is set to []. Set to 1 if using a control bedgraph output: - meta: type: map description: | Groovy Map containing sample information e.g. [ id:'test', single_end:false ] - bed: type: file description: Bed file containing the calculated peaks. pattern: "*.bed" - versions: type: file description: File containing software versions pattern: "versions.yml" authors: - "@chris-cheshire"