process DEEPTOOLS_BAMCOVERAGE { tag "$meta.id" label 'process_low' conda (params.enable_conda ? "bioconda::deeptools=3.5.1 bioconda::samtools=1.15.1" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/mulled-v2-eb9e7907c7a753917c1e4d7a64384c047429618a:2c687053c0252667cca265c9f4118f2c205a604c-0': 'quay.io/biocontainers/mulled-v2-eb9e7907c7a753917c1e4d7a64384c047429618a:2c687053c0252667cca265c9f4118f2c205a604c-0' }" input: tuple val(meta), path(input), path(input_index) path(fasta) path(fasta_fai) output: tuple val(meta), path("*.bigWig") , emit: bigwig, optional: true tuple val(meta), path("*.bedgraph") , emit: bedgraph, optional: true path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when script: def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}.bigWig" // cram_input is currently not working with deeptools // therefore it's required to convert cram to bam first def is_cram = input.Extension == "cram" ? true : false def input_out = is_cram ? input.BaseName + ".bam" : "${input}" def fai_reference = fasta_fai ? "--fai-reference ${fasta_fai}" : "" if (is_cram){ """ samtools view -T $fasta $input $fai_reference -@ $task.cpus -o $input_out samtools index -b $input_out -@ $task.cpus bamCoverage \\ --bam $input_out \\ $args \\ --numberOfProcessors ${task.cpus} \\ --outFileName ${prefix} cat <<-END_VERSIONS > versions.yml "${task.process}": samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') deeptools: \$(bamCoverage --version | sed -e "s/bamCoverage //g") END_VERSIONS """ } else { """ bamCoverage \\ --bam $input_out \\ $args \\ --numberOfProcessors ${task.cpus} \\ --outFileName ${prefix} cat <<-END_VERSIONS > versions.yml "${task.process}": deeptools: \$(bamCoverage --version | sed -e "s/bamCoverage //g") END_VERSIONS """ } }