#!/usr/bin/env nextflow nextflow.enable.dsl = 2 include { UNZIP as UNZIP_MALT } from '../../../modules/unzip/main.nf' addParams( options: [:] ) include { UNZIP as UNZIP_MALTEXTRACT } from '../../../modules/unzip/main.nf' addParams( options: [:] ) include { MALT_BUILD } from '../../../modules/malt/build/main.nf' addParams( options: [:] ) include { MALT_RUN } from '../../../modules/malt/run/main.nf' addParams( options: [:] ) include { MALTEXTRACT } from '../../../modules/maltextract/main.nf' addParams( options: [args: "-f def_anc"] ) include { AMPS } from '../../../modules/amps/main.nf' addParams( options: [:] ) workflow test_amps { fastas = file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) gff = [] seq_type = "DNA" map_db = file("https://software-ab.informatik.uni-tuebingen.de/download/megan6/megan-nucl-Jan2021.db.zip", checkIfExists: true) input = file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) mode = "BlastN" taxon_list = file(params.test_data['sarscov2']['genome']['taxon_list_txt'], checkIfExists: true) ncbi_dir = file(params.test_data['sarscov2']['genome']['ncbi_taxmap_zip'], checkIfExists: true) filter = "def_anc" UNZIP_MALT ( map_db ) UNZIP_MALTEXTRACT ( ncbi_dir ) MALT_BUILD ( fastas, seq_type, gff, UNZIP_MALT.out.unzipped_archive ) MALT_RUN ( input, mode, MALT_BUILD.out.index ) MALTEXTRACT ( MALT_RUN.out.rma6, taxon_list, UNZIP_MALTEXTRACT.out.unzipped_archive) AMPS ( MALTEXTRACT.out.results, taxon_list, filter ) }