// Import generic module functions include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' params.options = [:] options = initOptions(params.options) process FASTP { tag "$meta.id" label 'process_medium' publishDir "${params.outdir}", mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } conda (params.enable_conda ? 'bioconda::fastp=0.20.1' : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { container 'https://depot.galaxyproject.org/singularity/fastp:0.20.1--h8b12597_0' } else { container 'quay.io/biocontainers/fastp:0.20.1--h8b12597_0' } input: tuple val(meta), path(reads) val save_trimmed_fail val save_merged output: tuple val(meta), path('*.trim.fastq.gz') , emit: reads tuple val(meta), path('*.json') , emit: json tuple val(meta), path('*.html') , emit: html tuple val(meta), path('*.log') , emit: log path "versions.yml" , emit: version tuple val(meta), path('*.fail.fastq.gz') , optional:true, emit: reads_fail tuple val(meta), path('*.merged.fastq.gz'), optional:true, emit: reads_merged script: // Added soft-links to original fastqs for consistent naming in MultiQC def software = getSoftwareName(task.process) def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" if (meta.single_end) { def fail_fastq = save_trimmed_fail ? "--failed_out ${prefix}.fail.fastq.gz" : '' """ [ ! -f ${prefix}.fastq.gz ] && ln -s $reads ${prefix}.fastq.gz fastp \\ --in1 ${prefix}.fastq.gz \\ --out1 ${prefix}.trim.fastq.gz \\ --thread $task.cpus \\ --json ${prefix}.fastp.json \\ --html ${prefix}.fastp.html \\ $fail_fastq \\ $options.args \\ 2> ${prefix}.fastp.log cat <<-END_VERSIONS > versions.yml ${getProcessName(task.process)}: ${getSoftwareName(task.process)}: \$(fastp --version 2>&1 | sed -e "s/fastp //g") END_VERSIONS """ } else { def fail_fastq = save_trimmed_fail ? "--unpaired1 ${prefix}_1.fail.fastq.gz --unpaired2 ${prefix}_2.fail.fastq.gz" : '' def merge_fastq = save_merged ? "-m --merged_out ${prefix}.merged.fastq.gz" : '' """ [ ! -f ${prefix}_1.fastq.gz ] && ln -s ${reads[0]} ${prefix}_1.fastq.gz [ ! -f ${prefix}_2.fastq.gz ] && ln -s ${reads[1]} ${prefix}_2.fastq.gz fastp \\ --in1 ${prefix}_1.fastq.gz \\ --in2 ${prefix}_2.fastq.gz \\ --out1 ${prefix}_1.trim.fastq.gz \\ --out2 ${prefix}_2.trim.fastq.gz \\ --json ${prefix}.fastp.json \\ --html ${prefix}.fastp.html \\ $fail_fastq \\ $merge_fastq \\ --thread $task.cpus \\ --detect_adapter_for_pe \\ $options.args \\ 2> ${prefix}.fastp.log cat <<-END_VERSIONS > versions.yml ${getProcessName(task.process)}: ${getSoftwareName(task.process)}: \$(fastp --version 2>&1 | sed -e "s/fastp //g") END_VERSIONS """ } }