// Import generic module functions include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' params.options = [:] options = initOptions(params.options) params.use_cache = false params.snpeff_tag = "" process SNPEFF { label 'process_medium' publishDir "${params.outdir}", mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } conda (params.enable_conda ? "bioconda::snpeff=5.0" : null) if (params.use_cache) { if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { container "https://depot.galaxyproject.org/singularity/snpeff:5.0--hdfd78af_1" } else { container "quay.io/biocontainers/snpeff:5.0--hdfd78af_1" } } else { container "nfcore/snpeff:${params.snpeff_tag}" } input: tuple val(meta), path(vcf) val db path cache output: tuple val(meta), path("*.ann.vcf"), emit: vcf path "*.csv" , emit: report path "versions.yml" , emit: version script: def software = getSoftwareName(task.process) def avail_mem = 6 if (!task.memory) { log.info '[snpEff] Available memory not known - defaulting to 6GB. Specify process memory requirements to change this.' } else { avail_mem = task.memory.giga } def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" cache = params.use_cache ? "-dataDir \${PWD}/${snpeff_cache}" : "" """ snpEff -Xmx${avail_mem}g \\ $db \\ $options.args \\ -csvStats ${prefix}.csv \\ $cache \\ $vcf \\ > ${prefix}.ann.vcf cat <<-END_VERSIONS > versions.yml ${getProcessName(task.process)}: ${getSoftwareName(task.process)}: \$(snpEff -version 2>&1) END_VERSIONS """ }