#!/usr/bin/env nextflow nextflow.enable.dsl = 2 include { BOWTIE2_BUILD } from '../../../../modules/bowtie2/build/main.nf' include { BOWTIE2_ALIGN } from '../../../../modules/bowtie2/align/main.nf' workflow test_bowtie2_align_single_end { input = [ [ id:'test', single_end:true ], // meta map [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) ] ] fasta = file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) save_unaligned = false BOWTIE2_BUILD ( fasta ) BOWTIE2_ALIGN ( input, BOWTIE2_BUILD.out.index, save_unaligned ) } workflow test_bowtie2_align_paired_end { input = [ [ id:'test', single_end:false ], // meta map [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) ] ] fasta = file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) save_unaligned = false BOWTIE2_BUILD ( fasta ) BOWTIE2_ALIGN ( input, BOWTIE2_BUILD.out.index, save_unaligned ) } workflow test_bowtie2_align_single_end_large_index { input = [ [ id:'test', single_end:true ], // meta map [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) ] ] fasta = file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) save_unaligned = false BOWTIE2_BUILD ( fasta ) BOWTIE2_ALIGN ( input, BOWTIE2_BUILD.out.index, save_unaligned ) } workflow test_bowtie2_align_paired_end_large_index { input = [ [ id:'test', single_end:false ], // meta map [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) ] ] fasta = file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) save_unaligned = false BOWTIE2_BUILD ( fasta ) BOWTIE2_ALIGN ( input, BOWTIE2_BUILD.out.index, save_unaligned ) }