name: ivar_consensus description: Generate a consensus sequence from a BAM file using iVar keywords: - amplicon sequencing - consensus - fasta tools: - ivar: description: | iVar - a computational package that contains functions broadly useful for viral amplicon-based sequencing. homepage: https://github.com/andersen-lab/ivar documentation: https://andersen-lab.github.io/ivar/html/manualpage.html params: - outdir: type: string description: | The pipeline's output directory. By default, the module will output files into `$params.outdir/` - publish_dir_mode: type: string description: | Value for the Nextflow `publishDir` mode parameter. Available: symlink, rellink, link, copy, copyNoFollow, move. - enable_conda: type: boolean description: | Run the module with Conda using the software specified via the `conda` directive - singularity_pull_docker_container: type: boolean description: | Instead of directly downloading Singularity images for use with Singularity, force the workflow to pull and convert Docker containers instead. input: - meta: type: map description: | Groovy Map containing sample information e.g. [ id:'test', single_end:false ] - bam: type: file description: A sorted (with samtools sort) and trimmed (with iVar trim) bam file pattern: "*.bam" - ref: type: file description: The reference sequence used for mapping and generating the BAM file pattern: "*.fa" output: - meta: type: map description: | Groovy Map containing sample information e.g. [ id:'test', single_end:false ] - fasta: type: file description: iVar generated consensus sequence pattern: "*.fa" - qual: type: file description: iVar generated quality file pattern: "*.qual.txt" - mpileup: type: file description: mpileup output from samtools mpileup [OPTIONAL] pattern: "*.mpileup" - version: type: file description: File containing software version pattern: "*.{version.txt}" authors: - "@andersgs" - "@drpatelh"