// Import generic module functions include { initOptions; saveFiles; getSoftwareName } from './functions' params.options = [:] def options = initOptions(params.options) process IVAR_VARIANTS { tag "$meta.id" label 'process_medium' publishDir "${params.outdir}", mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), publish_id:meta.id) } conda (params.enable_conda ? "bioconda::ivar=1.3.1" : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { container "https://depot.galaxyproject.org/singularity/1.3.1--h089eab3_0" } else { container "quay.io/biocontainers/1.3.1--h089eab3_0" } input: tuple val(meta), path(bam) path fasta output: tuple val(meta), path("*.tsv"), emit: variants tuple val(meta), path("*.mpileup"), emit: mpileup path "*.version.txt" , emit: version script: def software = getSoftwareName(task.process) def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" def save_mpileup = params.containsKey('save_mpileup') ? "tee ${prefix}.mpileup |" : "" def gff = params.containsKey('gff') ? "-g ${params.get('gff')}" : "" """ samtools mpileup \\ $options.args2 \\ --reference $fasta \\ $bam | \\ $save_mpileup \\ ivar variants \\ $options.args \\ $gff \\ -r $fasta \\ -p $prefix ivar version | head -n1 2>&1 | sed 's/^.*iVar version //g' > ${software}.version.txt """ }