process GATK4_APPLYBQSR { tag "$meta.id" label 'process_low' conda (params.enable_conda ? "bioconda::gatk4=4.2.3.0" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/gatk4:4.2.3.0--hdfd78af_0' : 'quay.io/biocontainers/gatk4:4.2.3.0--hdfd78af_0' }" input: tuple val(meta), path(input), path(input_index), path(bqsr_table) path fasta path fai path dict path intervals output: tuple val(meta), path("*.bam"), emit: bam path "versions.yml" , emit: versions script: def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" def interval = intervals ? "-L ${intervals}" : "" if (!task.memory) { log.info '[GATK ApplyBQSR] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.' } else { avail_mem = task.memory.giga } """ gatk ApplyBQSR \\ -R $fasta \\ -I $input \\ --bqsr-recal-file $bqsr_table \\ $interval \\ --tmp-dir . \\ -O ${prefix}.bam \\ $args cat <<-END_VERSIONS > versions.yml "${task.process}": gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//') END_VERSIONS """ }