#!/usr/bin/env nextflow nextflow.enable.dsl = 2 include { GATK4_APPLYBQSR } from '../../../../modules/gatk4/applybqsr/main.nf' workflow test_gatk4_applybqsr { input = [ [ id:'test' ], // meta map file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true), file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam_bai'], checkIfExists: true), file(params.test_data['sarscov2']['illumina']['test_baserecalibrator_table'], checkIfExists: true) ] fasta = file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) fai = file(params.test_data['sarscov2']['genome']['genome_fasta_fai'], checkIfExists: true) dict = file(params.test_data['sarscov2']['genome']['genome_dict'], checkIfExists: true) GATK4_APPLYBQSR ( input, fasta, fai, dict, [] ) } workflow test_gatk4_applybqsr_intervals { input = [ [ id:'test' ], // meta map file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true), file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam_bai'], checkIfExists: true), file(params.test_data['sarscov2']['illumina']['test_baserecalibrator_table'], checkIfExists: true) ] fasta = file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) fai = file(params.test_data['sarscov2']['genome']['genome_fasta_fai'], checkIfExists: true) dict = file(params.test_data['sarscov2']['genome']['genome_dict'], checkIfExists: true) intervals = file(params.test_data['sarscov2']['genome']['test_bed'], checkIfExists: true) GATK4_APPLYBQSR ( input, fasta, fai, dict, intervals ) } workflow test_gatk4_applybqsr_cram { input = [ [ id:'test' ], // meta map file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_cram'], checkIfExists: true), file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_cram_crai'], checkIfExists: true), file(params.test_data['homo_sapiens']['illumina']['test_baserecalibrator_table'], checkIfExists: true) ] fasta = file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) fai = file(params.test_data['homo_sapiens']['genome']['genome_fasta_fai'], checkIfExists: true) dict = file(params.test_data['homo_sapiens']['genome']['genome_dict'], checkIfExists: true) intervals = file(params.test_data['homo_sapiens']['genome']['genome_bed'], checkIfExists: true) GATK4_APPLYBQSR ( input, fasta, fai, dict, intervals ) }