// Import generic module functions include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' params.options = [:] options = initOptions(params.options) process GENRICH { tag "$meta.id" label 'process_high' publishDir "${params.outdir}", mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } conda (params.enable_conda ? "bioconda::genrich=0.6.1" : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { container "https://depot.galaxyproject.org/singularity/genrich:0.6.1--h5bf99c6_1" } else { container "quay.io/biocontainers/genrich:0.6.1--h5bf99c6_1" } input: tuple val(meta), path(treatment_bam) path control_bam path blacklist_bed output: tuple val(meta), path("*narrowPeak") , emit: peaks tuple val(meta), path("*pvalues.bedGraph"), optional:true, emit: bedgraph_pvalues tuple val(meta), path("*pileup.bedGraph") , optional:true, emit: bedgraph_pileup tuple val(meta), path("*intervals.bed") , optional:true, emit: bed_intervals tuple val(meta), path("*duplicates.txt") , optional:true, emit: duplicates path "versions.yml" , emit: versions script: def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" def control = params.control_bam ? "-c $control_bam" : '' def pvalues = params.pvalues ? "-f ${prefix}.pvalues.bedGraph" : "" def pileup = params.pileup ? "-k ${prefix}.pileup.bedGraph" : "" def bed = params.bed ? "-b ${prefix}.intervals.bed" : "" def blacklist = params.blacklist_bed ? "-E $blacklist_bed" : "" def duplicates = "" if (params.save_duplicates) { if (options.args.contains('-r')) { duplicates = "-R ${prefix}.duplicates.txt" } else { log.info '[Genrich] Duplicates can only be saved if they are filtered, defaulting to -r option (Remove PCR duplicates).' duplicates = "-r -R ${prefix}.duplicates.txt" } } """ Genrich \\ -t $treatment_bam \\ $options.args \\ $control \\ $blacklist \\ -o ${prefix}.narrowPeak \\ $pvalues \\ $pileup \\ $bed \\ $duplicates \\ $blacklist \\ $control cat <<-END_VERSIONS > versions.yml ${getProcessName(task.process)}: ${getSoftwareName(task.process)}: \$(echo \$(Genrich --version 2>&1) | sed 's/^Genrich, version //; s/ .*\$//') END_VERSIONS """ }