// Import generic module functions include { initOptions; saveFiles; getSoftwareName } from './functions' process QUALIMAP_RNASEQ { tag "$meta.id" label 'process_medium' publishDir "${params.outdir}", mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:options, publish_dir:getSoftwareName(task.process), publish_id:meta.id) } container "quay.io/biocontainers/qualimap:2.2.2d--1" //container "https://depot.galaxyproject.org/singularity/qualimap:2.2.2d--1" conda (params.conda ? "bioconda::qualimap=2.2.2d" : null) input: tuple val(meta), path(bam) path gtf val options output: tuple val(meta), path("${prefix}"), emit: results path "*.version.txt" , emit: version script: def software = getSoftwareName(task.process) def ioptions = initOptions(options) prefix = ioptions.suffix ? "${meta.id}${ioptions.suffix}" : "${meta.id}" def paired_end = meta.single_end ? '' : '-pe' def memory = task.memory.toGiga() + "G" def strandedness = 'non-strand-specific' if (meta.strandedness == 'forward') { strandedness = 'strand-specific-forward' } else if (meta.strandedness == 'reverse') { strandedness = 'strand-specific-reverse' } """ unset DISPLAY mkdir tmp export _JAVA_OPTIONS=-Djava.io.tmpdir=./tmp qualimap \\ --java-mem-size=$memory \\ rnaseq \\ $ioptions.args \\ -bam $bam \\ -gtf $gtf \\ -p $strandedness \\ $paired_end \\ -outdir $prefix echo \$(qualimap 2>&1) | sed 's/^.*QualiMap v.//; s/Built.*\$//' > ${software}.version.txt """ }