process METAPHLAN3 { tag "$meta.id" label 'process_high' conda (params.enable_conda ? 'bioconda::metaphlan=3.0.12' : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/metaphlan:3.0.12--pyhb7b1952_0' : 'quay.io/biocontainers/metaphlan:3.0.12--pyhb7b1952_0' }" input: tuple val(meta), path(input) path metaphlan_db output: tuple val(meta), path("*_profile.txt") , emit: profile tuple val(meta), path("*.biom") , emit: biom tuple val(meta), path('*.bowtie2out.txt'), optional:true, emit: bt2out path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when script: def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" def input_type = ("$input".endsWith(".fastq.gz")) ? "--input_type fastq" : ("$input".contains(".fasta")) ? "--input_type fasta" : ("$input".endsWith(".bowtie2out.txt")) ? "--input_type bowtie2out" : "--input_type sam" def input_data = ("$input_type".contains("fastq")) && !meta.single_end ? "${input[0]},${input[1]}" : "$input" def bowtie2_out = "$input_type" == "--input_type bowtie2out" || "$input_type" == "--input_type sam" ? '' : "--bowtie2out ${prefix}.bowtie2out.txt" """ metaphlan \\ --nproc $task.cpus \\ $input_type \\ $input_data \\ $args \\ $bowtie2_out \\ --bowtie2db ${metaphlan_db} \\ --biom ${prefix}.biom \\ --output_file ${prefix}_profile.txt cat <<-END_VERSIONS > versions.yml "${task.process}": metaphlan3: \$(metaphlan --version 2>&1 | awk '{print \$3}') END_VERSIONS """ }