// Import generic module functions include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' params.options = [:] options = initOptions(params.options) process STRELKA_GERMLINE { tag "$meta.id" label 'process_high' publishDir "${params.outdir}", mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } conda (params.enable_conda ? "bioconda::strelka=2.9.10" : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { container "https://depot.galaxyproject.org/singularity/strelka:2.9.10--0" } else { container "quay.io/biocontainers/strelka:2.9.10--0" } input: tuple val(meta), path(input), path(input_index) path fasta path fai path target_bed path target_bed_tbi output: tuple val(meta), path("*variants.vcf.gz") , emit: vcf tuple val(meta), path("*variants.vcf.gz.tbi"), emit: vcf_tbi tuple val(meta), path("*genome.vcf.gz") , emit: genome_vcf tuple val(meta), path("*genome.vcf.gz.tbi") , emit: genome_vcf_tbi path "versions.yml" , emit: versions script: def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" def regions = target_bed ? "--exome --callRegions ${target_bed}" : "" """ configureStrelkaGermlineWorkflow.py \\ --bam $input \\ --referenceFasta $fasta \\ $regions \\ $options.args \\ --runDir strelka python strelka/runWorkflow.py -m local -j $task.cpus mv strelka/results/variants/genome.*.vcf.gz ${prefix}.genome.vcf.gz mv strelka/results/variants/genome.*.vcf.gz.tbi ${prefix}.genome.vcf.gz.tbi mv strelka/results/variants/variants.vcf.gz ${prefix}.variants.vcf.gz mv strelka/results/variants/variants.vcf.gz.tbi ${prefix}.variants.vcf.gz.tbi cat <<-END_VERSIONS > versions.yml ${getProcessName(task.process)}: ${getSoftwareName(task.process)}: \$( configureStrelkaGermlineWorkflow.py --version ) END_VERSIONS """ }