// Import generic module functions include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' params.options = [:] options = initOptions(params.options) process STRELKA_SOMATIC { tag "$meta.id" label 'process_high' publishDir "${params.outdir}", mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } conda (params.enable_conda ? "bioconda::strelka=2.9.10" : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { container "https://depot.galaxyproject.org/singularity/strelka:2.9.10--h9ee0642_1" } else { container "quay.io/biocontainers/strelka:2.9.10--h9ee0642_1" } input: tuple val(meta), path(cram_normal), path(crai_normal), path(cram_tumor), path(crai_tumor), path(manta_candidate_small_indels), path(manta_candidate_small_indels_tbi) path fasta path fai path target_bed path target_bed_tbi output: tuple val(meta), path("*.somatic_indels.vcf.gz") , emit: vcf_indels tuple val(meta), path("*.somatic_indels.vcf.gz.tbi"), emit: vcf_indels_tbi tuple val(meta), path("*.somatic_snvs.vcf.gz") , emit: vcf_snvs tuple val(meta), path("*.somatic_snvs.vcf.gz.tbi") , emit: vcf_snvs_tbi path "versions.yml" , emit: versions script: def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" def options_target_bed = target_bed ? "--exome --callRegions ${target_bed}" : "" def options_manta = manta_candidate_small_indels ? "--indelCandidates ${manta_candidate_small_indels}" : "" """ configureStrelkaSomaticWorkflow.py \\ --tumor $cram_tumor \\ --normal $cram_normal \\ --referenceFasta $fasta \\ $options_target_bed \\ $options_manta \\ $options.args \\ --runDir strelka python strelka/runWorkflow.py -m local -j $task.cpus mv strelka/results/variants/somatic.indels.vcf.gz ${prefix}.somatic_indels.vcf.gz mv strelka/results/variants/somatic.indels.vcf.gz.tbi ${prefix}.somatic_indels.vcf.gz.tbi mv strelka/results/variants/somatic.snvs.vcf.gz ${prefix}.somatic_snvs.vcf.gz mv strelka/results/variants/somatic.snvs.vcf.gz.tbi ${prefix}.somatic_snvs.vcf.gz.tbi cat <<-END_VERSIONS > versions.yml ${getProcessName(task.process)}: ${getSoftwareName(task.process)}: \$( configureStrelkaSomaticWorkflow.py --version ) END_VERSIONS """ }