// Import generic module functions include { initOptions; saveFiles; getSoftwareName } from './functions' params.options = [:] def options = initOptions(params.options) process QUALIMAP_BAMQC { tag "$meta.id" label 'process_medium' publishDir "${params.outdir}", mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), publish_id:meta.id) } conda (params.enable_conda ? "bioconda::qualimap=2.2.2d" : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { container "https://depot.galaxyproject.org/singularity/qualimap:2.2.2d--1" } else { container "quay.io/biocontainers/qualimap:2.2.2d--1" } input: tuple val(meta), path(bam) path gff val use_gff output: tuple val(meta), path("${prefix}"), emit: results path "*.version.txt" , emit: version script: def software = getSoftwareName(task.process) prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" def threads = task.cpus def collect_pairs = meta.single_end ? '' : '--collect-overlap-pairs' def memory = task.memory.toGiga() + "G" def regions = use_gff ? "--gff $gff" : '' def strandedness = 'non-strand-specific' if (meta.strandedness == 'forward') { strandedness = 'strand-specific-forward' } else if (meta.strandedness == 'reverse') { strandedness = 'strand-specific-reverse' } """ unset DISPLAY mkdir tmp export _JAVA_OPTIONS=-Djava.io.tmpdir=./tmp qualimap \\ --java-mem-size=$memory \\ bamqc \\ $options.args \\ -bam $bam \\ $regions \\ -p $strandedness \\ $collect_pairs \\ -outdir $prefix \\ -nt $threads echo \$(qualimap 2>&1) | sed 's/^.*QualiMap v.//; s/Built.*\$//' > ${software}.version.txt """ }