// Import generic module functions include { initOptions; saveFiles; getSoftwareName } from './functions' params.options = [:] options = initOptions(params.options) process SALMON_QUANT { tag "$meta.id" label "process_medium" publishDir "${params.outdir}", mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } conda (params.enable_conda ? 'bioconda::salmon=1.5.2' : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { container "https://depot.galaxyproject.org/singularity/salmon:1.5.2--h84f40af_0" } else { container "quay.io/biocontainers/salmon:1.5.2--h84f40af_0" } input: tuple val(meta), path(reads) path index path gtf path transcript_fasta val alignment_mode val lib_type output: tuple val(meta), path("${prefix}"), emit: results path "*.version.txt" , emit: version script: def software = getSoftwareName(task.process) prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" def reference = "--index $index" def input_reads = meta.single_end ? "-r $reads" : "-1 ${reads[0]} -2 ${reads[1]}" if (alignment_mode) { reference = "-t $transcript_fasta" input_reads = "-a $reads" } def strandedness_opts = [ 'A', 'U', 'SF', 'SR', 'IS', 'IU' , 'ISF', 'ISR', 'OS', 'OU' , 'OSF', 'OSR', 'MS', 'MU' , 'MSF', 'MSR' ] def strandedness = 'A' if (lib_type) { if (strandedness_opts.contains(lib_type)) { strandedness = lib_type } else { log.info "[Salmon Quant] Invalid library type specified '--libType=${lib_type}', defaulting to auto-detection with '--libType=A'." } } else { strandedness = meta.single_end ? 'U' : 'IU' if (meta.strandedness == 'forward') { strandedness = meta.single_end ? 'SF' : 'ISF' } else if (meta.strandedness == 'reverse') { strandedness = meta.single_end ? 'SR' : 'ISR' } } """ salmon quant \\ --geneMap $gtf \\ --threads $task.cpus \\ --libType=$strandedness \\ $reference \\ $input_reads \\ $options.args \\ -o $prefix salmon --version | sed -e "s/salmon //g" > ${software}.version.txt """ }