// TODO nf-core: If in doubt look at other nf-core/modules to see how we are doing things! :) // https://github.com/nf-core/modules/tree/master/modules // You can also ask for help via your pull request or on the #modules channel on the nf-core Slack workspace: // https://nf-co.re/join // TODO nf-core: A module file SHOULD only define input and output files as command-line parameters. // All other parameters MUST be provided using the "task.ext" directive, see here: // https://www.nextflow.io/docs/latest/process.html#ext // where "task.ext" is a string. // Any parameters that need to be evaluated in the context of a particular sample // e.g. single-end/paired-end data MUST also be defined and evaluated appropriately. // TODO nf-core: Software that can be piped together SHOULD be added to separate module files // unless there is a run-time, storage advantage in implementing in this way // e.g. it's ok to have a single module for bwa to output BAM instead of SAM: // bwa mem | samtools view -B -T ref.fasta // TODO nf-core: Optional inputs are not currently supported by Nextflow. However, using an empty // list (`[]`) instead of a file can be used to work around this issue. process EMBOSS_SEQRET { tag "$meta.id" label 'process_low' // TODO nf-core: List required Conda package(s). // Software MUST be pinned to channel (i.e. "bioconda"), version (i.e. "1.10"). // For Conda, the build (i.e. "h9402c20_2") must be EXCLUDED to support installation on different operating systems. // TODO nf-core: See section in main README for further information regarding finding and adding container addresses to the section below. conda (params.enable_conda ? "bioconda::emboss=6.6.0" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/emboss:6.6.0--hf657eab_5': 'quay.io/biocontainers/emboss:6.6.0--h440b012_4' }" input: // TODO nf-core: Where applicable all sample-specific information e.g. "id", "single_end", "read_group" // MUST be provided as an input via a Groovy Map called "meta". // This information may not be required in some instances e.g. indexing reference genome files: // https://github.com/nf-core/modules/blob/master/modules/bwa/index/main.nf // TODO nf-core: Where applicable please provide/convert compressed files as input/output // e.g. "*.fastq.gz" and NOT "*.fastq", "*.bam" and NOT "*.sam" etc. tuple val(meta), path(bam) output: // TODO nf-core: Named file extensions MUST be emitted for ALL output channels tuple val(meta), path("*.bam"), emit: bam // TODO nf-core: List additional required output channels/values here path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when script: def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" // TODO nf-core: Where possible, a command MUST be provided to obtain the version number of the software e.g. 1.10 // If the software is unable to output a version number on the command-line then it can be manually specified // e.g. https://github.com/nf-core/modules/blob/master/modules/homer/annotatepeaks/main.nf // Each software used MUST provide the software name and version number in the YAML version file (versions.yml) // TODO nf-core: It MUST be possible to pass additional parameters to the tool as a command-line string via the "task.ext.args" directive // TODO nf-core: If the tool supports multi-threading then you MUST provide the appropriate parameter // using the Nextflow "task" variable e.g. "--threads $task.cpus" // TODO nf-core: Please replace the example samtools command below with your module's command // TODO nf-core: Please indent the command appropriately (4 spaces!!) to help with readability ;) """ samtools \\ sort \\ $args \\ -@ $task.cpus \\ -o ${prefix}.bam \\ -T $prefix \\ $bam cat <<-END_VERSIONS > versions.yml "${task.process}": emboss: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//' )) END_VERSIONS """ }