name: fgbio_groupreadsbyumi description: | Groups reads together that appear to have come from the same original molecule. Reads are grouped by template, and then templates are sorted by the 5’ mapping positions of the reads from the template, used from earliest mapping position to latest. Reads that have the same end positions are then sub-grouped by UMI sequence. (!) Note: the MQ tag is required on reads with mapped mates (!) This can be added using samblaster with the optional argument --addMateTags. keywords: - UMI - groupreads - fgbio tools: - fgbio: description: A set of tools for working with genomic and high throughput sequencing data, including UMIs homepage: http://fulcrumgenomics.github.io/fgbio/ documentation: http://fulcrumgenomics.github.io/fgbio/tools/latest/ tool_dev_url: https://github.com/fulcrumgenomics/fgbio doi: "" licence: ["MIT"] input: - meta: type: map description: | Groovy Map containing sample information e.g. [ id:'test', single_end:false ] - bam: type: file description: | BAM file. Note: the MQ tag is required on reads with mapped mates (!) pattern: "*.bam" - strategy: type: value description: | Reguired argument: defines the UMI assignment strategy. Must be chosen among: Identity, Edit, Adjacency, Paired. output: - meta: type: map description: | Groovy Map containing sample information e.g. [ id:'test', single_end:false ] - versions: type: file description: File containing software versions pattern: "versions.yml" - bam: type: file description: UMI-grouped BAM pattern: "*.bam" - histogram: type: file description: A text file containing the tag family size counts pattern: "*.txt" authors: - "@lescai"