name: "atlas_call" description: generate VCF file from a BAM file using various calling methods keywords: - atlas - variant calling - vcf - population genetics tools: - "atlas": description: "ATLAS, a suite of methods to accurately genotype and estimate genetic diversity" homepage: "https://bitbucket.org/wegmannlab/atlas/wiki/Home" documentation: "https://bitbucket.org/wegmannlab/atlas/wiki/Home" tool_dev_url: "https://bitbucket.org/wegmannlab/atlas" doi: "10.1101/105346" licence: "['GPL v3']" input: - meta: type: map description: | Groovy Map containing sample information e.g. [ id:'test', single_end:false ] - bam: type: file description: A BAM/ file pattern: "*.bam" - bai: type: file description: The BAI file for the input BAM file pattern: "*.bai" - fasta: type: file description: The reference FASTA file used to generate the BAM file pattern: "*.{fasta,fna,fa}" - fai: type: file description: The index of the FASTA file used for to generate the BAM file pattern: "*.fai" - recal: type: file description: Optional recalibration file from atlas recal function in text format pattern: "*.txt" - pmd: type: file description: Optional PMD file from atlas pmd function in text format pattern: "*.txt" - known_alleles: type: file description: Optional tab separated text file containing 1-based list of known alleles. See atlas call documentation. pattern: "*.{txt.tsv}" - method: type: character description: Which variant calling algorithm to use. Some may require additional parameters supplied via ext.args. Check atlas documentation. pattern: "MLE|Bayesian|allelePresence|randomBase|majorityBase" output: - meta: type: map description: | Groovy Map containing sample information e.g. [ id:'test', single_end:false ] - versions: type: file description: File containing software versions pattern: "versions.yml" - bam: type: file description: VCF file with variant calls pattern: "*.vcf.gz" authors: - "@jfy133"