process VCFTOOLS { tag "$meta.id" label 'process_medium' conda (params.enable_conda ? "bioconda::vcftools=0.1.16" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/vcftools:0.1.16--he513fc3_4' : 'quay.io/biocontainers/vcftools:0.1.16--he513fc3_4' }" input: // Owing to the nature of vcftools we here provide solutions to working with optional bed files and optional // alternative variant files, for use with the 'diff' suite of tools. // Other optional input files can be utilised in a similar way to below but we do not exhaustively itterate through all // possible options. Instead we leave that to the user. tuple val(meta), path(variant_file) path bed path diff_variant_file output: tuple val(meta), path("*.vcf") , optional:true, emit: vcf tuple val(meta), path("*.bcf") , optional:true, emit: bcf tuple val(meta), path("*.frq") , optional:true, emit: frq tuple val(meta), path("*.frq.count") , optional:true, emit: frq_count tuple val(meta), path("*.idepth") , optional:true, emit: idepth tuple val(meta), path("*.ldepth") , optional:true, emit: ldepth tuple val(meta), path("*.ldepth.mean") , optional:true, emit: ldepth_mean tuple val(meta), path("*.gdepth") , optional:true, emit: gdepth tuple val(meta), path("*.hap.ld") , optional:true, emit: hap_ld tuple val(meta), path("*.geno.ld") , optional:true, emit: geno_ld tuple val(meta), path("*.geno.chisq") , optional:true, emit: geno_chisq tuple val(meta), path("*.list.hap.ld") , optional:true, emit: list_hap_ld tuple val(meta), path("*.list.geno.ld") , optional:true, emit: list_geno_ld tuple val(meta), path("*.interchrom.hap.ld") , optional:true, emit: interchrom_hap_ld tuple val(meta), path("*.interchrom.geno.ld") , optional:true, emit: interchrom_geno_ld tuple val(meta), path("*.TsTv") , optional:true, emit: tstv tuple val(meta), path("*.TsTv.summary") , optional:true, emit: tstv_summary tuple val(meta), path("*.TsTv.count") , optional:true, emit: tstv_count tuple val(meta), path("*.TsTv.qual") , optional:true, emit: tstv_qual tuple val(meta), path("*.FILTER.summary") , optional:true, emit: filter_summary tuple val(meta), path("*.sites.pi") , optional:true, emit: sites_pi tuple val(meta), path("*.windowed.pi") , optional:true, emit: windowed_pi tuple val(meta), path("*.weir.fst") , optional:true, emit: weir_fst tuple val(meta), path("*.het") , optional:true, emit: heterozygosity tuple val(meta), path("*.hwe") , optional:true, emit: hwe tuple val(meta), path("*.Tajima.D") , optional:true, emit: tajima_d tuple val(meta), path("*.ifreqburden") , optional:true, emit: freq_burden tuple val(meta), path("*.LROH") , optional:true, emit: lroh tuple val(meta), path("*.relatedness") , optional:true, emit: relatedness tuple val(meta), path("*.relatedness2") , optional:true, emit: relatedness2 tuple val(meta), path("*.lqual") , optional:true, emit: lqual tuple val(meta), path("*.imiss") , optional:true, emit: missing_individual tuple val(meta), path("*.lmiss") , optional:true, emit: missing_site tuple val(meta), path("*.snpden") , optional:true, emit: snp_density tuple val(meta), path("*.kept.sites") , optional:true, emit: kept_sites tuple val(meta), path("*.removed.sites") , optional:true, emit: removed_sites tuple val(meta), path("*.singletons") , optional:true, emit: singeltons tuple val(meta), path("*.indel.hist") , optional:true, emit: indel_hist tuple val(meta), path("*.hapcount") , optional:true, emit: hapcount tuple val(meta), path("*.mendel") , optional:true, emit: mendel tuple val(meta), path("*.FORMAT") , optional:true, emit: format tuple val(meta), path("*.INFO") , optional:true, emit: info tuple val(meta), path("*.012") , optional:true, emit: genotypes_matrix tuple val(meta), path("*.012.indv") , optional:true, emit: genotypes_matrix_individual tuple val(meta), path("*.012.pos") , optional:true, emit: genotypes_matrix_position tuple val(meta), path("*.impute.hap") , optional:true, emit: impute_hap tuple val(meta), path("*.impute.hap.legend") , optional:true, emit: impute_hap_legend tuple val(meta), path("*.impute.hap.indv") , optional:true, emit: impute_hap_indv tuple val(meta), path("*.ldhat.sites") , optional:true, emit: ldhat_sites tuple val(meta), path("*.ldhat.locs") , optional:true, emit: ldhat_locs tuple val(meta), path("*.BEAGLE.GL") , optional:true, emit: beagle_gl tuple val(meta), path("*.BEAGLE.PL") , optional:true, emit: beagle_pl tuple val(meta), path("*.ped") , optional:true, emit: ped tuple val(meta), path("*.map") , optional:true, emit: map_ tuple val(meta), path("*.tped") , optional:true, emit: tped tuple val(meta), path("*.tfam") , optional:true, emit: tfam tuple val(meta), path("*.diff.sites_in_files") , optional:true, emit: diff_sites_in_files tuple val(meta), path("*.diff.indv_in_files") , optional:true, emit: diff_indv_in_files tuple val(meta), path("*.diff.sites") , optional:true, emit: diff_sites tuple val(meta), path("*.diff.indv") , optional:true, emit: diff_indv tuple val(meta), path("*.diff.discordance.matrix"), optional:true, emit: diff_discd_matrix tuple val(meta), path("*.diff.switch") , optional:true, emit: diff_switch_error path "versions.yml" , emit: versions script: def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" def args_list = args.tokenize() def bed_arg = (args.contains('--bed')) ? "--bed ${bed}" : (args.contains('--exclude-bed')) ? "--exclude-bed ${bed}" : (args.contains('--hapcount')) ? "--hapcount ${bed}" : '' args_list.removeIf { it.contains('--bed') } args_list.removeIf { it.contains('--exclude-bed') } args_list.removeIf { it.contains('--hapcount') } def diff_variant_arg = (args.contains('--diff')) ? "--diff ${diff_variant_file}" : (args.contains('--gzdiff')) ? "--gzdiff ${diff_variant_file}" : (args.contains('--diff-bcf')) ? "--diff-bcf ${diff_variant_file}" : '' args_list.removeIf { it.contains('--diff') } args_list.removeIf { it.contains('--gzdiff') } args_list.removeIf { it.contains('--diff-bcf') } def input_file = ("$variant_file".endsWith(".vcf")) ? "--vcf ${variant_file}" : ("$variant_file".endsWith(".vcf.gz")) ? "--gzvcf ${variant_file}" : ("$variant_file".endsWith(".bcf")) ? "--bcf ${variant_file}" : '' """ vcftools \\ $input_file \\ --out $prefix \\ ${args_list.join(' ')} \\ $bed_arg \\ $diff_variant_arg cat <<-END_VERSIONS > versions.yml "${task.process}": vcftools: \$(echo \$(vcftools --version 2>&1) | sed 's/^.*VCFtools (//;s/).*//') END_VERSIONS """ }