process SUBREAD_FEATURECOUNTS { tag "$meta.id" label 'process_medium' conda (params.enable_conda ? "bioconda::subread=2.0.1" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/subread:2.0.1--hed695b0_0' : 'quay.io/biocontainers/subread:2.0.1--hed695b0_0' }" input: tuple val(meta), path(bams), path(annotation) output: tuple val(meta), path("*featureCounts.txt") , emit: counts tuple val(meta), path("*featureCounts.txt.summary"), emit: summary path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when script: def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" def paired_end = meta.single_end ? '' : '-p' def strandedness = 0 if (meta.strandedness == 'forward') { strandedness = 1 } else if (meta.strandedness == 'reverse') { strandedness = 2 } """ featureCounts \\ $args \\ $paired_end \\ -T $task.cpus \\ -a $annotation \\ -s $strandedness \\ -o ${prefix}.featureCounts.txt \\ ${bams.join(' ')} cat <<-END_VERSIONS > versions.yml "${task.process}": subread: \$( echo \$(featureCounts -v 2>&1) | sed -e "s/featureCounts v//g") END_VERSIONS """ }