// Import generic module functions include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' params.options = [:] options = initOptions(params.options) process RSEM_PREPAREREFERENCE { tag "$fasta" label 'process_high' publishDir "${params.outdir}", mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'index', meta:[:], publish_by_meta:[]) } conda (params.enable_conda ? "bioconda::rsem=1.3.3 bioconda::star=2.7.6a" : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { container "https://depot.galaxyproject.org/singularity/mulled-v2-cf0123ef83b3c38c13e3b0696a3f285d3f20f15b:606b713ec440e799d53a2b51a6e79dbfd28ecf3e-0" } else { container "quay.io/biocontainers/mulled-v2-cf0123ef83b3c38c13e3b0696a3f285d3f20f15b:606b713ec440e799d53a2b51a6e79dbfd28ecf3e-0" } input: path fasta, stageAs: "rsem/*" path gtf output: path "rsem" , emit: index path "rsem/*transcripts.fa", emit: transcript_fasta path "versions.yml" , emit: version script: def software = getSoftwareName(task.process) def args = options.args.tokenize() if (args.contains('--star')) { args.removeIf { it.contains('--star') } def memory = task.memory ? "--limitGenomeGenerateRAM ${task.memory.toBytes() - 100000000}" : '' """ STAR \\ --runMode genomeGenerate \\ --genomeDir rsem/ \\ --genomeFastaFiles $fasta \\ --sjdbGTFfile $gtf \\ --runThreadN $task.cpus \\ $memory \\ $options.args2 rsem-prepare-reference \\ --gtf $gtf \\ --num-threads $task.cpus \\ ${args.join(' ')} \\ $fasta \\ rsem/genome cat <<-END_VERSIONS > versions.yml ${getProcessName(task.process)}: ${getSoftwareName(task.process)}: \$(rsem-calculate-expression --version | sed -e "s/Current version: RSEM v//g") star: \$(STAR --version | sed -e "s/STAR_//g") END_VERSIONS """ } else { """ rsem-prepare-reference \\ --gtf $gtf \\ --num-threads $task.cpus \\ $options.args \\ $fasta \\ rsem/genome cat <<-END_VERSIONS > versions.yml ${getProcessName(task.process)}: ${getSoftwareName(task.process)}: \$(rsem-calculate-expression --version | sed -e "s/Current version: RSEM v//g") star: \$(STAR --version | sed -e "s/STAR_//g") END_VERSIONS """ } }