// Import generic module functions include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' params.options = [:] options = initOptions(params.options) process SUBREAD_FEATURECOUNTS { tag "$meta.id" label 'process_medium' publishDir "${params.outdir}", mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } conda (params.enable_conda ? "bioconda::subread=2.0.1" : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { container "https://depot.galaxyproject.org/singularity/subread:2.0.1--hed695b0_0" } else { container "quay.io/biocontainers/subread:2.0.1--hed695b0_0" } input: tuple val(meta), path(bams), path(annotation) output: tuple val(meta), path("*featureCounts.txt") , emit: counts tuple val(meta), path("*featureCounts.txt.summary"), emit: summary path "versions.yml" , emit: version script: def software = getSoftwareName(task.process) def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" def paired_end = meta.single_end ? '' : '-p' def strandedness = 0 if (meta.strandedness == 'forward') { strandedness = 1 } else if (meta.strandedness == 'reverse') { strandedness = 2 } """ featureCounts \\ $options.args \\ $paired_end \\ -T $task.cpus \\ -a $annotation \\ -s $strandedness \\ -o ${prefix}.featureCounts.txt \\ ${bams.join(' ')} cat <<-END_VERSIONS > versions.yml ${getProcessName(task.process)}: ${getSoftwareName(task.process)}: \$( echo \$(featureCounts -v 2>&1) | sed -e "s/featureCounts v//g") END_VERSIONS """ }