process GATK4_MARKDUPLICATES { tag "$meta.id" label 'process_low' conda (params.enable_conda ? "bioconda::gatk4=4.2.4.1" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/gatk4:4.2.4.1--hdfd78af_0' : 'quay.io/biocontainers/gatk4:4.2.4.1--hdfd78af_0' }" input: tuple val(meta), path(bams) output: tuple val(meta), path("*.bam") , emit: bam tuple val(meta), path("*.bai") , emit: bai tuple val(meta), path("*.metrics"), emit: metrics path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when script: def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" def bam_list = bams.collect(){ bam -> "--INPUT ".concat(bam.toString()) }.join(" ") def avail_mem = 3 if (!task.memory) { log.info '[GATK MarkDuplicates] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.' } else { avail_mem = task.memory.giga } """ gatk --java-options "-Xmx${avail_mem}g" MarkDuplicates \\ $bam_list \\ --METRICS_FILE ${prefix}.metrics \\ --TMP_DIR . \\ --CREATE_INDEX true \\ --OUTPUT ${prefix}.bam \\ $args cat <<-END_VERSIONS > versions.yml "${task.process}": gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//') END_VERSIONS """ }